CFCM · LABORATORY PRACTICE

Troubleshooting

Start with the problem you observe. Connect instrument setup, sample preparation, panel design and interpretation with existing CFCM learning material.

Find the relevant learning path

Unexpected populations and staining

Explore the troubleshooting curriculum: dead cells, autofluorescence, Fc binding, staining artefacts and gating errors.

Compensation and spreading

Start with panel design, fluorochromes and controls. Open the curriculum outline.

Instrument setup and abnormal scatter

Find the instrument setup and QC learning material.

Sample quality and laboratory workflow

Explore the laboratory quality curriculum.

Changed assay performance

Connect troubleshooting with validation and verification.

Interpretation and detection

Read existing evidence-linked Clinical Notes.

Keep the evidence connected

Academy · Resources · Intelligence · Clinical Cases — in preparation

Explore the four practical troubleshooting guides below, alongside related material and curriculum outlines.

Return to CFCM

Apply the controls to a clinical question

Use the same artifact reasoning when a restrictive AML gate, a discordant B-cell phenotype or a low-event MRD finding needs explanation.